Real-time probing in vivo RNA structure and interactions by RNA-based synthetic structure sensors
Etablissement : Université Paris Descartes
Promotion : 2016
Domaine d’étude : Sciences de la vie et de la santé
Unité de recherche : INSERM U1001
Ecole doctorale : Frontières du Vivant (FdV)
Description de la thèse :
RNA not only conveys the genetic information and but also performs as a key regulatory factor in the biological systems. Its function mostly relies on the structural characters and dynamics. We aim to develop a new methodology, using synthetic RNA system called “structure sensor” for real-time probing in vivo. The structure sensor is designed based on the RNA-RNA interaction, i.e. nucleic acid hybridization, consisting of a probe, a switch and a reporter.